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Engineering viruses to deliver gene therapy to targeted cell populations

Tatyana Dobreva
David Brown

Adeno-associated viruses are used to deliver gene therapy for genetic diseases, but they often have trouble reaching certain parts of the body; we work on methods for engineering the virus protein shell to reach hard-to-reach cell types, like neurons in the brain, and for better understanding where and how these viruses operate.

Viruses can be humanity's Trojan horses when it comes to reaching hard to get places in the body and delivering a therapeutic, like gene therapy. In our case, we are interested in different neuronal populations in the brain. We work with colleagues in Viviana Gradinaru's group at Caltech to process and analyze sequencing data recovered from mouse brain tissue samples that have been injected with custom engineered adeno-associated viruses. We are investigating what components of the virus capsid contribute to making it past the blood-brain barrier and into specific types of cells, and seeking to better to understand their functional fingerprint. Some elements of our work include:

  • Analyzing next-generation sequencing datasets to quantify relative transduction efficiency of large libraries of viral variants

  • Performing machine learning classification and regression of libraries of viral variants to find patterns in enrichment and cell-type specificity and make predictions on new variants

  • Using unsupervised clustering methods to find classes of viral variants with similar properties

  • Developing single-cell sequencing methodologies to understand, at a deeper level, the behavior of these viruses

High-Throughput Method Speeds Discovery of Improved Vectors For Gene Delivery To Diverse Brain Cell Types

Using deep sequencing to guide evolution of AAVs has yielded rich datasets, and new viral variants, such as one that is better able to transduce brain vasculature

Delivering Genes Across the Blood-Brain Barrier

Caltech biologists have developed a vector capable of noninvasive delivery of genetic cargo throughout the adult central nervous system.

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